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MRPL14 Polyclonal antibody

MRPL14 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15040-1-AP

Print datasheet

Synonyms

MRP-L14, MRP L32, MRP L14, Large ribosomal subunit protein uL14m, L32mt

验证数据展示

  • WB analysis of HEK-293 using 15040-1-AP

    WB analysis of HEK-293 using 15040-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15040-1-AP (MRPL14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • IF Staining of HEK-293 using 15040-1-AP

    IF Staining of HEK-293 using 15040-1-AP

    Immunofluorescent analysis of HEK-293 cells using 15040-1-AP (MRPL14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15040-1-AP targets MRPL14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPL14 fusion protein Ag6808 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L14
Synonyms MRP-L14, MRP L32, MRP L14, Large ribosomal subunit protein uL14m, L32mt
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC065005
Gene Symbol MRPL14
Gene ID (NCBI) 64928
RRIDAB_2878102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P1L8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL14 antibody 15040-1-APDownload protocol
IF protocol for MRPL14 antibody 15040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
HUMANWB

EMBO Mol Med

SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.

Authors - Alexandre Janer
View Article