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验证数据展示

  • WB analysis using 14762-1-AP

    WB analysis using 14762-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14762-1-AP (MRPL16 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14762-1-AP

    IP experiment of HeLa using 14762-1-AP

    IP result of anti-MRPL16 (IP:14762-1-AP, 4ug; Detection:14762-1-AP 1:500) with HeLa cells lysate 1520 ug.

  • IHC staining of human intrahepatic cholangiocarcinoma using 14762-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 14762-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14762-1-AP (MRPL16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL16 Polyclonal antibody

MRPL16 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14762-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

PNAS-111, PNAS 111, MRP-L16, MRP L16, Large ribosomal subunit protein uL16m

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14762-1-AP targets MRPL16 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL16 fusion protein Ag6523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L16
别名 PNAS-111, PNAS 111, MRP-L16, MRP L16, Large ribosomal subunit protein uL16m
计算分子量 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC001040
基因名称 MRPL16
Gene ID (NCBI) 54948
RRIDAB_3085442
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NX20
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPL16 (Mitochondrial Ribosomal Protein L16) is a protein coding gene. It encodes a 39S subunit protein. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. The molecular mass of MRPL16 is 28 kDa.

实验方案

Product Specific Protocols
WB protocol for MRPL16 antibody 14762-1-APDownload protocol
IHC protocol for MRPL16 antibody 14762-1-APDownload protocol
IP protocol for MRPL16 antibody 14762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols