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验证数据展示

  • WB analysis of HepG2 using 15178-1-AP

    WB analysis of HepG2 using 15178-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15178-1-AP (MRPL18 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 15178-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 15178-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15178-1-AP (MRPL18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL18 Polyclonal antibody

MRPL18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15178-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MRP-L18, MRPL 18, MRP L18, Large ribosomal subunit protein uL18m, L18mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15178-1-AP targets MRPL18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL18 fusion protein Ag7096 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L18
别名 MRP-L18, MRPL 18, MRP L18, Large ribosomal subunit protein uL18m, L18mt
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC001623
基因名称 MRPL18
Gene ID (NCBI) 29074
RRIDAB_2878114
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0U6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL18 antibody 15178-1-APDownload protocol
IHC protocol for MRPL18 antibody 15178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols