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MRPL19 Polyclonal antibody

MRPL19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16517-1-AP

Print datasheet

Synonyms

MRP L19, MRP L15, Large ribosomal subunit protein bL19m, L19mt, L15mt

验证数据展示

  • WB analysis of mouse kidney using 16517-1-AP

    WB analysis of mouse kidney using 16517-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16517-1-AP (MRPL19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 16517-1-AP

    IF Staining of HeLa using 16517-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16517-1-AP (MRPL19 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach cancer using 16517-1-AP

    IHC staining of human stomach cancer using 16517-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16517-1-AP (MRPL19 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16517-1-AP targets MRPL19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MRPL19 fusion protein Ag9738 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L19
Synonyms MRP L19, MRP L15, Large ribosomal subunit protein bL19m, L19mt, L15mt
Calculated Molecular Weight 292 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC030144
Gene Symbol MRPL19
Gene ID (NCBI) 9801
RRIDAB_2250806
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49406
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL19 antibody 16517-1-APDownload protocol
IHC protocol for MRPL19 antibody 16517-1-APDownload protocol
IF protocol for MRPL19 antibody 16517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Transl Lung Cancer Res

Overexpression of MRPL19 in predicting poor prognosis and promoting the development of lung adenocarcinoma

Authors - Dong Wei
View Article