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MRPL22 Polyclonal antibody

MRPL22 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16299-1-AP

Print datasheet

Synonyms

MRPL 22, MRP L25, MRP L22, L25mt, L22mt

验证数据展示

  • WB analysis of PC-3 using 16299-1-AP

    WB analysis of PC-3 using 16299-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16299-1-AP (MRPL22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 16299-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 16299-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16299-1-AP (MRPL22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16299-1-AP targets MRPL22 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MRPL22 fusion protein Ag8899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L22
Synonyms MRPL 22, MRP L25, MRP L22, L25mt, L22mt
Calculated Molecular Weight 206 aa, 24 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC012565
Gene Symbol MRPL22
Gene ID (NCBI) 29093
RRIDAB_2810293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL22 antibody 16299-1-APDownload protocol
IHC protocol for MRPL22 antibody 16299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols