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验证数据展示

  • WB analysis of PC-3 using 16299-1-AP

    WB analysis of PC-3 using 16299-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16299-1-AP (MRPL22 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 16299-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 16299-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16299-1-AP (MRPL22 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL22 Polyclonal antibody

MRPL22 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16299-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

MRPL 22, MRP L25, MRP L22, L25mt, L22mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16299-1-AP targets MRPL22 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL22 fusion protein Ag8899 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L22
别名 MRPL 22, MRP L25, MRP L22, L25mt, L22mt
计算分子量 206 aa, 24 kDa
观测分子量 20 kDa
GenBank蛋白编号BC012565
基因名称 MRPL22
Gene ID (NCBI) 29093
RRIDAB_2810293
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL22 antibody 16299-1-APDownload protocol
IHC protocol for MRPL22 antibody 16299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols