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MRPL23 Monoclonal antibody

MRPL23 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G11C4

Cat No : 66595-1-Ig

Print datasheet

Synonyms

L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L

验证数据展示

  • WB analysis of HEK-293 using 66595-1-Ig

    WB analysis of HEK-293 using 66595-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66595-1-Ig (MRPL23 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66595-1-Ig

    IHC staining of human breast cancer using 66595-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66595-1-Ig (MRPL23 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66595-1-Ig

    IHC staining of human breast cancer using 66595-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66595-1-Ig (MRPL23 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 66595-1-Ig

    IF Staining of HEK-293 using 66595-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 66595-1-Ig (MRPL23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66595-1-Ig targets MRPL23 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MRPL23 fusion protein Ag2220 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name mitochondrial ribosomal protein L23
Synonyms L23MRP, L23mt, MRP L23, MRPL23, Ribosomal protein L23 like, RPL23, RPL23L
Calculated Molecular Weight 153 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC027710
Gene Symbol MRPL23
Gene ID (NCBI) 6150
RRIDAB_2881955
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL23 antibody 66595-1-IgDownload protocol
IHC protocol for MRPL23 antibody 66595-1-IgDownload protocol
IF protocol for MRPL23 antibody 66595-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols