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MRPL28 Polyclonal antibody

MRPL28 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21604-1-AP

Print datasheet

Synonyms

L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15

验证数据展示

  • WB analysis using 21604-1-AP

    WB analysis using 21604-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21604-1-AP (MRPL28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 21604-1-AP

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 21604-1-AP

    IHC staining of human kidney using 21604-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21604-1-AP (MRPL28 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 21604-1-AP

    IF Staining of HeLa using 21604-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL28 antibody (21604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse brain using 21604-1-AP

    IP experiment of mouse brain using 21604-1-AP

    IP result of anti-MRPL28 (IP:21604-1-AP, 4ug; Detection:21604-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, JAR cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 21604-1-AP is MRPL28 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPL28 fusion protein Ag16224 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L28
Synonyms L28mt, MAAT1, Melanoma antigen p15, MRP L28, MRPL28, p15
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC000990
Gene Symbol MRPL28
Gene ID (NCBI) 10573
RRIDAB_10859785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL28 antibody 21604-1-APDownload protocol
IHC protocol for MRPL28 antibody 21604-1-APDownload protocol
IF protocol for MRPL28 antibody 21604-1-APDownload protocol
IP protocol for MRPL28 antibody 21604-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article
humanWB

J Ethnopharmacol

Elucidating the pharmacological effects of Compound Kushen injection on MYC-P15-CCND1 signaling pathway in nasopharyngeal carcinoma - An in vitro study

Authors - Zhishan Wu
View Article