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MRPL34 Polyclonal antibody

MRPL34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15233-1-AP

Print datasheet

Synonyms

MRP-L34, MRP L34, Large ribosomal subunit protein bL34m, L34mt

验证数据展示

  • WB analysis using 15233-1-AP

    WB analysis using 15233-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15233-1-AP (MRPL34 antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 15233-1-AP

    IHC staining of mouse lung using 15233-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15233-1-AP (MRPL34 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 15233-1-AP

    IHC staining of mouse lung using 15233-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 15233-1-AP (MRPL34 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 15233-1-AP

    IF Staining of HepG2 using 15233-1-AP

    Immunofluorescent analysis of HepG2 cells using 15233-1-AP (MRPL34 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15233-1-AP targets MRPL34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MRPL34 fusion protein Ag7173 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L34
Synonyms MRP-L34, MRP L34, Large ribosomal subunit protein bL34m, L34mt
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC000071
Gene Symbol MRPL34
Gene ID (NCBI) 64981
RRIDAB_2145915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL34 antibody 15233-1-APDownload protocol
IHC protocol for MRPL34 antibody 15233-1-APDownload protocol
IF protocol for MRPL34 antibody 15233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols