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MRPL38 Polyclonal antibody

MRPL38 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 15913-1-AP

Print datasheet

Synonyms

MRP L38, MRP L3, Large ribosomal subunit protein mL38, L38mt, KIAA1863

验证数据展示

  • WB analysis using 15913-1-AP

    WB analysis using 15913-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15913-1-AP (MRPL38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15913-1-AP

    IHC staining of human liver using 15913-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15913-1-AP (MRPL38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HEK-293 using 15913-1-AP

    FC experiment of HEK-293 using 15913-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.5 ug MRPL38 Polyclonal antibody (15913-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15913-1-AP targets MRPL38 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen MRPL38 fusion protein Ag8706 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L38
Synonyms MRP L38, MRP L3, Large ribosomal subunit protein mL38, L38mt, KIAA1863
Calculated Molecular Weight 346 aa, 41 kDa
Observed Molecular Weight37-45 kDa
GenBank Accession NumberBC013311
Gene Symbol MRPL38
Gene ID (NCBI) 64978
RRIDAB_2146047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DV4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL38 antibody 15913-1-APDownload protocol
IHC protocol for MRPL38 antibody 15913-1-APDownload protocol
FC protocol for MRPL38 antibody 15913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Translation Fidelity and Respiration Deficits in CLPP-Deficient Tissues: Mechanistic Insights from Mitochondrial Complexome Profiling

Authors - Jana Key
View Article
ratWB

Cell Rep

Small RNAs derived from tRNA fragmentation regulate the functional maturation of neonatal β cells.

Authors - Mustafa Bilal Bayazit
View Article
IHC

J Toxicol Sci

Aberrant epigenetic gene regulation in hippocampal neurogenesis of mouse offspring following maternal exposure to 3,3'-iminodipropionitrile.

Authors - Takeshi Tanaka
View Article