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MRPL39 Polyclonal antibody

MRPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28165-1-AP

Print datasheet

Synonyms

C21orf92, FLJ20451, L39mt, L5mt, MRP L39, MRP L5, MRPL39, MRPL5, MSTP003, PRED22, PRED66, RPML5

验证数据展示

  • WB analysis using 28165-1-AP

    WB analysis using 28165-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28165-1-AP (MRPL39 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 28165-1-AP

    IHC staining of human liver using 28165-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28165-1-AP (MRPL39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28165-1-AP

    IF Staining of HepG2 using 28165-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 28165-1-AP (MRPL39 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, mouse heart tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

28165-1-AP targets MRPL39 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman
Immunogen MRPL39 fusion protein Ag27154 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L39
Synonyms C21orf92, FLJ20451, L39mt, L5mt, MRP L39, MRP L5, MRPL39, MRPL5, MSTP003, PRED22, PRED66, RPML5
Calculated Molecular Weight 338 aa, 39 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC107719
Gene Symbol MRPL39
Gene ID (NCBI) 54148
RRIDAB_2881080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NYK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL39 antibody 28165-1-APDownload protocol
IHC protocol for MRPL39 antibody 28165-1-APDownload protocol
IF protocol for MRPL39 antibody 28165-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Genet

Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency

Authors - Shabnam Bakhshalizadeh
View Article
humanWB

Signal Transduct Target Ther

The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.

Authors - Xujun Liu
View Article
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article