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验证数据展示

  • WB analysis using 17300-1-AP

    WB analysis using 17300-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17300-1-AP (MRPL42 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 17300-1-AP

    IHC staining of human breast cancer using 17300-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17300-1-AP (MRPL42 antibody) at dilution of 1:1000 (under 10x lens).

    Ready-to-use IHC kits available with MRPL42 antibody included
  • IF Staining of HeLa using 17300-1-AP

    IF Staining of HeLa using 17300-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPL42 antibody (17300-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MRPL42 Polyclonal antibody

MRPL42 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17300-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

MRP L42, MRP L31, ML42, L42mt, L31mt

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy MRPL42 Ready-To-Use IHC Kit. MRPL42 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17300-1-AP targets MRPL42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 MRPL42 fusion protein Ag11181 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L42
别名 MRP L42, MRP L31, ML42, L42mt, L31mt
计算分子量 142 aa, 17 kDa
观测分子量 14-17 kDa
GenBank蛋白编号BC040240
基因名称 MRPL42
Gene ID (NCBI) 28977
RRIDAB_2935442
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6G3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL42 antibody 17300-1-APDownload protocol
IHC protocol for MRPL42 antibody 17300-1-APDownload protocol
IF protocol for MRPL42 antibody 17300-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols