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MRPL43 Polyclonal antibody

MRPL43 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17477-1-AP

Print datasheet

Synonyms

bMRP36a, L43mt, MRP L43, MRPL43

验证数据展示

  • WB analysis using 17477-1-AP

    WB analysis using 17477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17477-1-AP (MRPL43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 17477-1-AP

    IHC staining of mouse skeletal muscle using 17477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17477-1-AP (MRPL43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17477-1-AP targets MRPL43 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MRPL43 fusion protein Ag11498 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L43
Synonyms bMRP36a, L43mt, MRP L43, MRPL43
Calculated Molecular Weight159aa,18 kDa; 215aa,23 kDa
Observed Molecular Weight17-23 kDa
GenBank Accession NumberBC015905
Gene Symbol MRPL43
Gene ID (NCBI) 84545
RRIDAB_2923567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N983
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL43 antibody 17477-1-APDownload protocol
IHC protocol for MRPL43 antibody 17477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols