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验证数据展示

  • WB analysis using 17477-1-AP

    WB analysis using 17477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17477-1-AP (MRPL43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 17477-1-AP

    IHC staining of mouse skeletal muscle using 17477-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17477-1-AP (MRPL43 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRPL43 Polyclonal antibody

MRPL43 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17477-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

bMRP36a, L43mt, MRP L43, MRPL43

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17477-1-AP targets MRPL43 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL43 fusion protein Ag11498 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L43
别名 bMRP36a, L43mt, MRP L43, MRPL43
计算分子量159aa,18 kDa; 215aa,23 kDa
观测分子量17-23 kDa
GenBank蛋白编号BC015905
基因名称 MRPL43
Gene ID (NCBI) 84545
RRIDAB_2923567
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N983
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL43 antibody 17477-1-APDownload protocol
IHC protocol for MRPL43 antibody 17477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols