验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, SKOV-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15682-1-AP targets MRPL45 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MRPL45 fusion protein Ag8184 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial ribosomal protein L45 |
| 别名 | MRP-L45, Large ribosomal subunit protein mL45, L45mt, 39S ribosomal protein L45, mitochondrial |
| 计算分子量 | 35 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC006235 |
| 基因名称 | MRPL45 |
| Gene ID (NCBI) | 84311 |
| RRID | AB_2146065 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BRJ2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| IHC protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| IF protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| IP protocol for MRPL45 antibody 15682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. | ||
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
Proc Natl Acad Sci U S A Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria. | ||
J Cell Biol FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function. |
