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  • Featured Product
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MRPL45 Polyclonal antibody

MRPL45 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15682-1-AP

Print datasheet

Synonyms

MRP-L45, Large ribosomal subunit protein mL45, L45mt, 39S ribosomal protein L45, mitochondrial

验证数据展示

  • WB analysis using 15682-1-AP

    WB analysis using 15682-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15682-1-AP (MRPL45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 15682-1-AP

    IHC staining of human ovary tumor using 15682-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15682-1-AP (MRPL45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 15682-1-AP

    WB analysis of HeLa using 15682-1-AP

    WB result of MRPL45 antibody (15682-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells.

  • IP experiment of HeLa using 15682-1-AP

    IP experiment of HeLa using 15682-1-AP

    IP result of anti-MRPL45 (IP:15682-1-AP, 4ug; Detection:15682-1-AP 1:1000) with HeLa cells lysate 680 ug.

  • IHC staining of mouse brain using 15682-1-AP

    IHC staining of mouse brain using 15682-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15682-1-AP (MRPL45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 15682-1-AP

    IF Staining of HeLa using 15682-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MRPL45 antibody (15682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15682-1-AP targets MRPL45 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MRPL45 fusion protein Ag8184 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L45
Synonyms MRP-L45, Large ribosomal subunit protein mL45, L45mt, 39S ribosomal protein L45, mitochondrial
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC006235
Gene Symbol MRPL45
Gene ID (NCBI) 84311
RRIDAB_2146065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.

实验方案

Product Specific Protocols
WB protocol for MRPL45 antibody 15682-1-APDownload protocol
IHC protocol for MRPL45 antibody 15682-1-APDownload protocol
IF protocol for MRPL45 antibody 15682-1-APDownload protocol
IP protocol for MRPL45 antibody 15682-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
View Article
mouseWB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
View Article
humanWB

Nat Commun

RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis.

Authors - Uwe Richter
View Article
humanIF

Proc Natl Acad Sci U S A

Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.

Authors - Gokhan Akman
View Article
humanWB

J Cell Biol

FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.

Authors - Liang Wang
View Article