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  • Featured Product
  • KD/KO Validated

MRPL45 Monoclonal antibody, PBS Only

MRPL45 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4B4

Cat no : 66531-1-PBS

Print datasheet

Synonyms

L45mt, MRPL45, MRP-L45

验证数据展示

  • IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

产品信息

66531-1-PBS targets MRPL45 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen MRPL45 fusion protein Ag8207 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitochondrial ribosomal protein L45
Synonyms L45mt, MRPL45, MRP-L45
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC006235
Gene Symbol MRPL45
Gene ID (NCBI) 84311
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRJ2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.