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验证数据展示

  • IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

    WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

MRPL45 Monoclonal antibody, PBS Only

MRPL45 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66531-1-PBS

产品说明书

CloneNo. 1B4B4

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

L45mt, MRPL45, MRP-L45

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66531-1-PBS targets MRPL45 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 MRPL45 fusion protein Ag8207 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L45
别名 L45mt, MRPL45, MRP-L45
计算分子量 35 kDa
观测分子量 33 kDa
GenBank蛋白编号BC006235
基因名称 MRPL45
Gene ID (NCBI) 84311
RRIDAB_2881894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRJ2
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.