验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IF | See 1 publications below |
产品信息
14677-1-AP targets MRPL48 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, bovine |
| 免疫原 | MRPL48 fusion protein Ag6387 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial ribosomal protein L48 |
| 别名 | Large ribosomal subunit protein mL48, L48mt, HSPC290, CGI-118, CGI118 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 20-21 kDa |
| GenBank蛋白编号 | BC009764 |
| 基因名称 | MRPL48 |
| Gene ID (NCBI) | 51642 |
| RRID | AB_2282151 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96GC5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRPL48, also named as CGI-118, or HSPC290 is a 212 amino acid protein, which localizes in the Mitochondrion. MRPL48 is a component of the large subunit of the mitochondrial ribosome, including two RNA molecules and, at least, 70 proteins among which 47 proteins for the large subunit. mitochondrial ribosomal protein helps in protein synthesis within the mitochondrion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MRPL48 antibody 14677-1-AP | Download protocol |
| IHC protocol for MRPL48 antibody 14677-1-AP | Download protocol |
| IF protocol for MRPL48 antibody 14677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function. | ||
Nucleic Acids Res Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis. | ||
Int J Mol Sci Low Expression of Mitofusin 1 Gene Leads to Mitochondrial Dysfunction and Embryonic Genome Activation Failure in Ovine-Bovine Inter-Species Cloned Embryos | ||
Cell Rep MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. | ||
Biomedicines Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema |
