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MRPL52 Polyclonal antibody

MRPL52 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16800-1-AP

Print datasheet

Synonyms

L52mt, MRP L52, MRPL52

验证数据展示

  • IHC staining of human kidney using 16800-1-AP

    IHC staining of human kidney using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 16800-1-AP

    IHC staining of human kidney using 16800-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 16800-1-AP

    IF Staining of HepG2 using 16800-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16800-1-AP (MRPL52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inhuman kidney tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16800-1-AP targets MRPL52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MRPL52 fusion protein Ag10280 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L52
Synonyms L52mt, MRP L52, MRPL52
Calculated Molecular Weight 123 aa, 14 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC068070
Gene Symbol MRPL52
Gene ID (NCBI) 122704
RRIDAB_2250853
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86TS9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL52 antibody 16800-1-APDownload protocol
IHC protocol for MRPL52 antibody 16800-1-APDownload protocol
IF protocol for MRPL52 antibody 16800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

J Oral Pathol Med

LncRNA LINC00152 promotes oral squamous cell carcinoma growth via enhancing Upstream Transcription Factor 1 mediated Mitochondrial Ribosomal Protein L52 transcription.

Authors - Xiuhe Zou
View Article