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验证数据展示

  • WB analysis of A549 using 16142-1-AP

    WB analysis of A549 using 16142-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 16142-1-AP (MRPL53 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 16142-1-AP

    IHC staining of human skin cancer using 16142-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16142-1-AP (MRPL53 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin cancer using 16142-1-AP

    IHC staining of human skin cancer using 16142-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16142-1-AP (MRPL53 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A549 using 16142-1-AP

    IF Staining of A549 using 16142-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 16142-1-AP (MRPL53 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MRPL53 Polyclonal antibody

MRPL53 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16142-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

L53mt, MRP L53, MRPL53

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16142-1-AP targets MRPL53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MRPL53 fusion protein Ag9210 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein L53
别名 L53mt, MRP L53, MRPL53
计算分子量 112 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC012163
基因名称 MRPL53
Gene ID (NCBI) 116540
RRIDAB_2878223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96EL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL53 antibody 16142-1-APDownload protocol
IHC protocol for MRPL53 antibody 16142-1-APDownload protocol
IF protocol for MRPL53 antibody 16142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols