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MRPL54 Polyclonal antibody

MRPL54 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17683-1-AP

Print datasheet

Synonyms

MRP-L54, MRP L54, Large ribosomal subunit protein mL54, L54mt

验证数据展示

  • WB analysis of mouse liver using 17683-1-AP

    WB analysis of mouse liver using 17683-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17683-1-AP (MRPL54 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 17683-1-AP

    IHC staining of human skin cancer using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin cancer using 17683-1-AP

    IHC staining of human skin cancer using 17683-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17683-1-AP (MRPL54 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 17683-1-AP

    IF Staining of HeLa using 17683-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17683-1-AP (MRPL54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman skin cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17683-1-AP targets MRPL54 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MRPL54 fusion protein Ag11782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L54
Synonyms MRP-L54, MRP L54, Large ribosomal subunit protein mL54, L54mt
Calculated Molecular Weight 138 aa, 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC065273
Gene Symbol MRPL54
Gene ID (NCBI) 116541
RRIDAB_2878429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P161
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL54 antibody 17683-1-APDownload protocol
IHC protocol for MRPL54 antibody 17683-1-APDownload protocol
IF protocol for MRPL54 antibody 17683-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols