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MRPL9 Polyclonal antibody

MRPL9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 15342-1-AP

Print datasheet

Synonyms

MRP-L9, MRP L9, Large ribosomal subunit protein bL9m, L9mt

验证数据展示

  • WB analysis using 15342-1-AP

    WB analysis using 15342-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15342-1-AP (MRPL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15342-1-AP

    IHC staining of human breast cancer using 15342-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15342-1-AP (MRPL9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15342-1-AP

    IF Staining of MCF-7 using 15342-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 15342-1-AP (MRPL9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15342-1-AP targets MRPL9 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPL9 fusion protein Ag7545 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein L9
Synonyms MRP-L9, MRP L9, Large ribosomal subunit protein bL9m, L9mt
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 26-28 kDa
GenBank Accession NumberBC004517
Gene Symbol MRPL9
Gene ID (NCBI) 65005
RRIDAB_2146214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYD2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPL9 antibody 15342-1-APDownload protocol
IHC protocol for MRPL9 antibody 15342-1-APDownload protocol
IF protocol for MRPL9 antibody 15342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Genet

Deficiency of the mitochondrial ribosomal subunit, MRPL50, causes autosomal recessive syndromic premature ovarian insufficiency

Authors - Shabnam Bakhshalizadeh
View Article
humanWB

Front Surg

Classification of the mitochondrial ribosomal protein-associated molecular subtypes and identified a serological diagnostic biomarker in hepatocellular carcinoma

Authors - Cong Xie
View Article
humanWB,IHC,IF,CoIP

Int J Mol Sci

Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer

Authors - Hui-Min Zhang
View Article
humanWB

Hum Mol Genet

Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease

Authors - Sumudu S C Amarasekera
View Article