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MRPS14 Polyclonal antibody

MRPS14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16301-1-AP

Print datasheet

Synonyms

HSMRPS14, MRP S14, MRPS14, S14mt

验证数据展示

  • WB analysis of mouse liver using 16301-1-AP

    WB analysis of mouse liver using 16301-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16301-1-AP (MRPS14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16301-1-AP

    IHC staining of mouse liver using 16301-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16301-1-AP (MRPS14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

16301-1-AP targets MRPS14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPS14 fusion protein Ag9018 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S14
Synonyms HSMRPS14, MRP S14, MRPS14, S14mt
Calculated Molecular Weight 128 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009788
Gene Symbol MRPS14
Gene ID (NCBI) 63931
RRIDAB_2878240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60783
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS14 antibody 16301-1-APDownload protocol
IHC protocol for MRPS14 antibody 16301-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
View Article
humanWB

Nat Commun

Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling.

Authors - Hauke S Hillen
View Article
humanWB

Nucleic Acids Res

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Authors - Elena Lavdovskaia
View Article
humanWB

Elife

Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.

Authors - Sven Dennerlein
View Article