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MRPS16 Polyclonal antibody

MRPS16 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Cat no : 16735-1-AP

Print datasheet

Synonyms

MRPS 16, MRP S16, COXPD2, CGI-132, CGI132

验证数据展示

WB analysis using 16735-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16735-1-AP (MRPS16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16735-1-AP
WB result of MRPS16 antibody (16735-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS16 transfected HeLa cells.
IP experiment of HEK-293 using 16735-1-AP
IP result of anti-MRPS16 (IP:16735-1-AP, 4ug; Detection:16735-1-AP 1:500) with HEK-293 cells lysate 1480ug.
IF Staining of HepG2 using 16735-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16735-1-AP (MRPS16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 16735-1-AP

Size

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经过测试的应用

Positive WB detected in	HEK-293 cells, HeLa cells, Jurkat cells, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HEK-293 cells
Positive IF/ICC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 18 publications below

产品信息

16735-1-AP targets MRPS16 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IF/ICC, IP, ELISA Application Description
Cited Applications	WB
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Immunogen	MRPS16 fusion protein Ag9749 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	mitochondrial ribosomal protein S16
Synonyms	MRPS 16, MRP S16, COXPD2, CGI-132, CGI132
Calculated Molecular Weight	137 aa, 15 kDa
Observed Molecular Weight	15 kDa
GenBank Accession Number	BC021106
Gene Symbol	MRPS16
Gene ID (NCBI)	51021
RRID	AB_2180166
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y3D3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS16 antibody 16735-1-AP	Download protocol
IF protocol for MRPS16 antibody 16735-1-AP	Download protocol
IP protocol for MRPS16 antibody 16735-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Sci Adv Adult-onset obesity is triggered by impaired mitochondrial gene expression. Authors - Kara L Perks View Article
mouse	WB	Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. Authors - Danielle L Rudler View Article
human	WB	Nucleic Acids Res The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis. Authors - Elena Lavdovskaia View Article
mouse	WB	Nat Commun Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice. Authors - Tara R Richman View Article
mouse	WB	EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. Authors - Nicola Ferreira View Article
mouse	WB	Aging Cell Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan. Authors - Tara R Richman View Article

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研究领域

MRPS16 Polyclonal antibody

MRPS16 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

验证数据展示

WB analysis using 16735-1-AP

WB analysis of HeLa using 16735-1-AP

IP experiment of HEK-293 using 16735-1-AP

IF Staining of HepG2 using 16735-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.

Loss of the RNA-binding protein TACO1 causes late-onset mitochondrial dysfunction in mice.

Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation.

Mitochondrial mistranslation modulated by metabolic stress causes cardiovascular disease and reduced lifespan.