MRPS17 Polyclonal antibody

MRPS17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18881-1-AP

Print datasheet

Synonyms

HSPC011, MRP S17, MRPS17, RPMS17, S17mt



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18881-1-AP targets MRPS17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MRPS17 fusion protein Ag13414 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S17
Synonyms HSPC011, MRP S17, MRPS17, RPMS17, S17mt
Calculated Molecular Weight 130 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC054031
Gene Symbol MRPS17
Gene ID (NCBI) 51373
RRIDAB_10597844
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2R5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS17 antibody 18881-1-APDownload protocol
IHC protocol for MRPS17 antibody 18881-1-APDownload protocol
IF protocol for MRPS17 antibody 18881-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria

Authors - Sabine Poerschke
humanWB

Nucleic Acids Res

METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis.

Authors - Lindsey Van Haute
humanWB

Nucleic Acids Res

Transcription-independent role for human mitochondrial RNA polymerase in mitochondrial ribosome biogenesis.

Authors - Surovtseva Yulia V YV
humanWB

Nucleic Acids Res

MPV17L2 is required for ribosome assembly in mitochondria.

Authors - Ilaria Dalla Rosa
humanWB

PLoS Genet

RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases.

Authors - Aurelio Reyes
humanWB

RNA Biol

TRMT2B is responsible for both tRNA and rRNA m5U-methylation in human mitochondria.

Authors - Christopher A Powell