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验证数据展示

  • WB analysis using 16235-1-AP

    WB analysis using 16235-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16235-1-AP (MRPS18A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16235-1-AP

    WB analysis of HepG2 using 16235-1-AP

    WB result of MRPS18A antibody (16235-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS18A transfected HepG2 cells.

  • IHC staining of human stomach using 16235-1-AP

    IHC staining of human stomach using 16235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16235-1-AP (MRPS18A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 16235-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 16235-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16235-1-AP (MRPS18A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

MRPS18A Polyclonal antibody

MRPS18A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16235-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

MRP S18 a, MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16235-1-AP targets MRPS18A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen MRPS18A fusion protein Ag8981 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S18A
Synonyms MRP S18 a, MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial
Calculated Molecular Weight 196 aa, 22 kDa
Observed Molecular Weight 18-22 kDa
GenBank Accession NumberBC010364
Gene Symbol MRPS18A
Gene ID (NCBI) 55168
RRIDAB_2266663
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVS2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).

实验方案

Product Specific Protocols
WB protocol for MRPS18A antibody 16235-1-APDownload protocol
IHC protocol for MRPS18A antibody 16235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Exp Oncol

Expression pattern of MRPS18 family genes in malignantly transformed b-cells.

Authors - L Kovalevska
View Article