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验证数据展示

  • WB analysis using 16235-1-AP

    WB analysis using 16235-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16235-1-AP (MRPS18A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 16235-1-AP

    WB analysis of HepG2 using 16235-1-AP

    WB result of MRPS18A antibody (16235-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS18A transfected HepG2 cells.

  • IHC staining of human stomach using 16235-1-AP

    IHC staining of human stomach using 16235-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16235-1-AP (MRPS18A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human intrahepatic cholangiocarcinoma using 16235-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 16235-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16235-1-AP (MRPS18A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

MRPS18A Polyclonal antibody

MRPS18A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16235-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MRP S18 a, MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16235-1-AP targets MRPS18A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC
经测试反应性 human
文献引用反应性human
免疫原 MRPS18A fusion protein Ag8981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitochondrial ribosomal protein S18A
别名 MRP S18 a, MRP S18 3, Large ribosomal subunit protein bS18a, HumanS18b, 39S ribosomal protein S18a, mitochondrial
计算分子量 196 aa, 22 kDa
观测分子量 18-22 kDa
GenBank蛋白编号BC010364
基因名称 MRPS18A
Gene ID (NCBI) 55168
RRIDAB_2266663
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NVS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MRPS18A (Mitochondrial Ribosomal Protein S18A) is a protein coding gene. It encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The increased expression of MRPS18A in cancer cells is explained by the increased energy metabolism of cancer cells and may pave the way for new diagnostic and therapeutic avenues to be explored (PMID: 28056857).

实验方案

Product Specific Protocols
WB protocol for MRPS18A antibody 16235-1-APDownload protocol
IHC protocol for MRPS18A antibody 16235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Exp Oncol

Expression pattern of MRPS18 family genes in malignantly transformed b-cells.

Authors - L Kovalevska
View Article