验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, 4T1 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67903-1-Ig targets MRPS25 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | WB, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | MRPS25 fusion protein Ag7846 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | mitochondrial ribosomal protein S25 |
Synonyms | DKFZp313H0817, FLJ00023, MRP S25, MRPS25, RPMS25, S25mt |
Calculated Molecular Weight | 20 kDa |
Observed Molecular Weight | 20 kDa |
GenBank Accession Number | BC003590 |
Gene Symbol | MRPS25 |
Gene ID (NCBI) | 64432 |
RRID | AB_2918659 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P82663 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitochondrial ribosomal protein S25 (MRPS25) belongs to the ribosomal protein S25/L51 family. It is a component of the mitochondrial ribosome small subunit (28S). The variant segregated with the disease and substitutes a highly conserved proline residue with leucine (p.P72L) that, based on the high-resolution structure of the 28S ribosome, is predicted to compromise inter-protein contacts and destabilize the small subunit.
实验方案
Product Specific Protocols | |
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WB protocol for MRPS25 antibody 67903-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |