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MRPS27 Monoclonal antibody, PBS Only

MRPS27 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1B3

Cat no : 66724-1-PBS

Print datasheet

Synonyms

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

验证数据展示

  • WB analysis of LNCaP using 66724-1-Ig (same clone as 66724-1-PBS)

    WB analysis of LNCaP using 66724-1-Ig (same clone as 66724-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

    IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

    IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

产品信息

66724-1-PBS targets MRPS27 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen MRPS27 fusion protein Ag11047 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name mitochondrial ribosomal protein S27
Synonyms FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt
Calculated Molecular Weight 168 aa, 19 kDa, 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC030521
Gene Symbol MRPS27
Gene ID (NCBI) 23107
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92552
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].