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MRPS28 Polyclonal antibody

MRPS28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16378-1-AP

Print datasheet

Synonyms

MRPS35, MRP-S28, MRPS 28, MRP S35, MRP S28

验证数据展示

  • WB analysis using 16378-1-AP

    WB analysis using 16378-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16378-1-AP (MRPS28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16378-1-AP

    IF Staining of HepG2 using 16378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16378-1-AP (MRPS28 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver using 16378-1-AP

    IHC staining of human liver using 16378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MRPS28 antibody included

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy MRPS28 Ready-To-Use IHC Kit. MRPS28 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16378-1-AP targets MRPS28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPS28 fusion protein Ag9354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S28
Synonyms MRPS35, MRP-S28, MRPS 28, MRP S35, MRP S28
Calculated Molecular Weight 187 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC010150
Gene Symbol MRPS28
Gene ID (NCBI) 28957
RRIDAB_2180748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Q9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS28 antibody 16378-1-APDownload protocol
IHC protocol for MRPS28 antibody 16378-1-APDownload protocol
IF protocol for MRPS28 antibody 16378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Authors - Juliette Pulman
View Article