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MRPS34 Polyclonal antibody

MRPS34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15166-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein mS34, S34mt, MRP-S34, MRPS12, MRP S34

验证数据展示

  • WB analysis using 15166-1-AP

    WB analysis using 15166-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15166-1-AP (MRPS34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15166-1-AP

    IHC staining of human breast cancer using 15166-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15166-1-AP (MRPS34 antibody) at dilution of 1:500 (under 10x lens).

    Ready-to-use IHC kits available with MRPS34 antibody included
  • IF Staining of A549 using 15166-1-AP

    IF Staining of A549 using 15166-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MRPS34 antibody (15166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy MRPS34 Ready-To-Use IHC Kit. MRPS34 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15166-1-AP targets MRPS34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MRPS34 fusion protein Ag7287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S34
Synonyms Small ribosomal subunit protein mS34, S34mt, MRP-S34, MRPS12, MRP S34
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC001182
Gene Symbol MRPS34
Gene ID (NCBI) 65993
RRIDAB_2144233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS34 antibody 15166-1-APDownload protocol
IHC protocol for MRPS34 antibody 15166-1-APDownload protocol
IF protocol for MRPS34 antibody 15166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols