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MRPS34 Polyclonal antibody

MRPS34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15166-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein mS34, S34mt, MRP-S34, MRPS12, MRP S34

验证数据展示

  • WB analysis using 15166-1-AP

    WB analysis using 15166-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15166-1-AP (MRPS34 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 15166-1-AP

    IHC staining of human breast cancer using 15166-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15166-1-AP (MRPS34 antibody) at dilution of 1:500 (under 10x lens).

    Ready-to-use IHC kits available with MRPS34 antibody included
  • IF Staining of A549 using 15166-1-AP

    IF Staining of A549 using 15166-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MRPS34 antibody (15166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy MRPS34 Ready-To-Use IHC Kit. MRPS34 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15166-1-AP targets MRPS34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen MRPS34 fusion protein Ag7287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S34
Synonyms Small ribosomal subunit protein mS34, S34mt, MRP-S34, MRPS12, MRP S34
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC001182
Gene Symbol MRPS34
Gene ID (NCBI) 65993
RRIDAB_2144233
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82930
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS34 antibody 15166-1-APDownload protocol
IHC protocol for MRPS34 antibody 15166-1-APDownload protocol
IF protocol for MRPS34 antibody 15166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

iScience

Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation

Authors - Taru Hilander
View Article