• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

MRPS5 Polyclonal antibody

MRPS5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16428-1-AP

Print datasheet

Synonyms

MRP S5, MRPS5, S5mt

验证数据展示

  • WB analysis using 16428-1-AP

    WB analysis using 16428-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16428-1-AP (MRPS5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16428-1-AP

    WB analysis of HeLa using 16428-1-AP

    WB result of MRPS5 antibody (16428-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS5 transfected HeLa cells.

  • IHC staining of human kidney using 16428-1-AP

    IHC staining of human kidney using 16428-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16428-1-AP (MRPS5 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 16428-1-AP

    IHC staining of human kidney using 16428-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16428-1-AP (MRPS5 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

16428-1-AP targets MRPS5 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen MRPS5 fusion protein Ag9672 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S5
Synonyms MRP S5, MRPS5, S5mt
Calculated Molecular Weight 430 aa, 48 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC014172
Gene Symbol MRPS5
Gene ID (NCBI) 64969
RRIDAB_2878258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82675
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS5 antibody 16428-1-APDownload protocol
IHC protocol for MRPS5 antibody 16428-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Transl Oncol

Porphyromonas gingivalis promotes malignancy and chemo-resistance via GSK3β-mediated mitochondrial oxidative phosphorylation in human esophageal squamous cell carcinoma

Authors - Yiwen Liu
View Article