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MRPS9 Polyclonal antibody

MRPS9 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16533-1-AP

Print datasheet

Synonyms

Small ribosomal subunit protein uS9m, S9mt, RPMS9, MRP-S9, MRP S9

验证数据展示

  • WB analysis using 16533-1-AP

    WB analysis using 16533-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16533-1-AP (MRPS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16533-1-AP

    WB analysis of HEK-293 using 16533-1-AP

    WB result of MRPS9 antibody (16533-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPS9 transfected HEK-293 cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 16533-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 16533-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16533-1-AP (MRPS9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16533-1-AP targets MRPS9 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen MRPS9 fusion protein Ag9809 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S9
Synonyms Small ribosomal subunit protein uS9m, S9mt, RPMS9, MRP-S9, MRP S9
Calculated Molecular Weight 396 aa, 46 kDa
Observed Molecular Weight 38-45 kDa
GenBank Accession NumberBC057240
Gene Symbol MRPS9
Gene ID (NCBI) 64965
RRIDAB_2878272
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS9 antibody 16533-1-APDownload protocol
IHC protocol for MRPS9 antibody 16533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

C17orf80 binds the mitochondrial genome to promote its replication

Authors - Hao Wu
View Article