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MS4A12 Polyclonal antibody

MS4A12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13293-1-AP

Print datasheet

Synonyms

Membrane-spanning 4-domains subfamily A member 12

验证数据展示

  • WB analysis using 13293-1-AP

    WB analysis using 13293-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13293-1-AP

    WB analysis using 13293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13293-1-AP

    IHC staining of human colon cancer using 13293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

  • IHC staining of human colon cancer using 13293-1-AP

    IHC staining of human colon cancer using 13293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13293-1-AP targets MS4A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MS4A12 fusion protein Ag3968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name membrane-spanning 4-domains, subfamily A, member 12
Synonyms Membrane-spanning 4-domains subfamily A member 12
Calculated Molecular Weight 267 aa, 28 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC029793
Gene Symbol MS4A12
Gene ID (NCBI) 54860
RRIDAB_10596915
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MS4A12 antibody 13293-1-APDownload protocol
IHC protocol for MS4A12 antibody 13293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols