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验证数据展示

  • WB analysis using 13293-1-AP

    WB analysis using 13293-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13293-1-AP

    WB analysis using 13293-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13293-1-AP (MS4A12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13293-1-AP

    IHC staining of human colon cancer using 13293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

  • IHC staining of human colon cancer using 13293-1-AP

    IHC staining of human colon cancer using 13293-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13293-1-AP (MS4A12 Antibody) at dilution of 1:50.

MS4A12 Polyclonal antibody

MS4A12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13293-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Membrane-spanning 4-domains subfamily A member 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13293-1-AP targets MS4A12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MS4A12 fusion protein Ag3968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 membrane-spanning 4-domains, subfamily A, member 12
别名 Membrane-spanning 4-domains subfamily A member 12
计算分子量 267 aa, 28 kDa
观测分子量 35 kDa
GenBank蛋白编号BC029793
基因名称 MS4A12
Gene ID (NCBI) 54860
RRIDAB_10596915
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXJ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MS4A12 antibody 13293-1-APDownload protocol
IHC protocol for MS4A12 antibody 13293-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols