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MSC Polyclonal antibody

MSC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25028-1-AP

Print datasheet

Synonyms

bHLHa22, Activated B-cell factor 1, Activated B cell factor 1, ABF-1, ABF1

验证数据展示

  • WB analysis of mouse spleen using 25028-1-AP

    WB analysis of mouse spleen using 25028-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25028-1-AP (MSC Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 25028-1-AP

    IHC staining of human lymphoma using 25028-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25028-1-AP (MSC Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse spleen tissue, mouse thymus tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25028-1-AP targets MSC in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen MSC fusion protein Ag13489 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name musculin (activated B-cell factor-1)
Synonyms bHLHa22, Activated B-cell factor 1, Activated B cell factor 1, ABF-1, ABF1
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC006313
Gene Symbol MSC
Gene ID (NCBI) 9242
RRIDAB_2879857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60682
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSC, also named as Musculin, is a 206 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain. MSC localizes in the nucleus and as a transcription repressor is capable of inhibiting the transactivation capability of TCF3/E47. MSC may play a role in regulating antigen-dependent B-cell differentiation.

实验方案

Product Specific Protocols
WB protocol for MSC antibody 25028-1-APDownload protocol
IHC protocol for MSC antibody 25028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols