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验证数据展示

  • Sandwich ELISA standard curve of MP50235-1

    Sandwich ELISA standard curve of MP50235-1

    Sandwich ELISA standard curve of MP50235-1, Human MSH2 Monoclonal Matched Antibody Pair - PBS only. 60161-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7835. 60161-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

  • Sample test of MP50235-1

    Sample test of MP50235-1

    The mean MSH2 concentration was determined to be 10.60 ng/mL in A375 cell extract based on a 2.0 mg/mL extract load and 17.80 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load.

  • IHC staining of Ramos using 60161-2-Ig (same clone as 60161-2-PBS)

    IHC staining of Ramos using 60161-2-Ig (same clone as 60161-2-PBS)

    Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human tonsillitis using 60161-2-Ig (same clone as 60161-2-PBS)

    IHC staining of human tonsillitis using 60161-2-Ig (same clone as 60161-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60161-2-Ig (MSH2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MSH2 antibody included
  • Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1

    Cytometric bead array standard curve of MP50235-1, MSH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60161-1-PBS. Detection antibody: 60161-2-PBS. Standard:Ag7835. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50235-2

    Cytometric bead array standard curve of MP50235-2

    Cytometric bead array standard curve of MP50235-2, MSH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60161-2-PBS. Detection antibody: 60161-3-PBS. Standard:Ag7835. Range: 0.098-100 ng/mL.

MSH2 Monoclonal antibody, PBS Only (Capture/Detector)

MSH2 Monoclonal Antibody for IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 60161-2-PBS

产品说明书

CloneNo. 2E4A7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

2E4A7, MSH 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.

产品信息

60161-2-PBS targets MSH2 as part of a matched antibody pair:

MP50235-1: 60161-1-PBS capture and 60161-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP50235-2: 60161-2-PBS capture and 60161-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 MSH2 fusion protein Ag7835 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
别名 2E4A7, MSH 2
计算分子量 105 kDa
观测分子量105 kDa
GenBank蛋白编号BC021566
基因名称 MSH2
Gene ID (NCBI) 4436
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A Magarose purification
UNIPROT IDP43246
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.