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MSMP Polyclonal antibody

MSMP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 19526-1-AP

Print datasheet

Synonyms

MSMP, PC 3, PC3 secreted microprotein, PSMP

验证数据展示

  • WB analysis using 19526-1-AP

    WB analysis using 19526-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 19526-1-AP

    WB analysis of DU 145 using 19526-1-AP

    WB result of MSMP antibody (19526-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSMP transfected DU 145 cells.

  • WB analysis using 19526-1-AP

    WB analysis using 19526-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 19526-1-AP

    IF Staining of PC-3 using 19526-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MSMP antibody (19526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, DU 145 cells
Positive IF/ICC detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19526-1-AP targets MSMP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name microseminoprotein, prostate associated
Synonyms MSMP, PC 3, PC3 secreted microprotein, PSMP
Calculated Molecular Weight 15 kDa
Observed Molecular Weight12 kDa, 20 kDa
GenBank Accession NumberNM_001044264
Gene Symbol MSMP
Gene ID (NCBI) 692094
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ1L6U9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSMP, also named PSMP, belongs to the beta-microseminoprotein family. MSMP is highly conserved, sharing 91 to 94% amino acid identity with its rodent and dog homologs. RT-PCR of 27 human tissues detected PSMP expression only in trachea and, to a lesser extent, testis. MSMP was highly expressed in PC3 cells, but not in 2 other prostate cancer cell lines. Western blot analysis revealed 20 kDa and 12 kDa bands PSMP maybe due to posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for MSMP antibody 19526-1-APDownload protocol
IF protocol for MSMP antibody 19526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols