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验证数据展示

  • WB analysis using 19526-1-AP

    WB analysis using 19526-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 19526-1-AP

    WB analysis of DU 145 using 19526-1-AP

    WB result of MSMP antibody (19526-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSMP transfected DU 145 cells.

  • WB analysis using 19526-1-AP

    WB analysis using 19526-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19526-1-AP (MSMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 19526-1-AP

    IF Staining of PC-3 using 19526-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using MSMP antibody (19526-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

MSMP Polyclonal antibody

MSMP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 19526-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

MSMP, PC 3, PC3 secreted microprotein, PSMP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, DU 145 cells
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19526-1-AP targets MSMP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 microseminoprotein, prostate associated
别名 MSMP, PC 3, PC3 secreted microprotein, PSMP
计算分子量 15 kDa
观测分子量12 kDa, 20 kDa
GenBank蛋白编号NM_001044264
基因名称 MSMP
Gene ID (NCBI) 692094
RRIDAB_3085579
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ1L6U9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSMP, also named PSMP, belongs to the beta-microseminoprotein family. MSMP is highly conserved, sharing 91 to 94% amino acid identity with its rodent and dog homologs. RT-PCR of 27 human tissues detected PSMP expression only in trachea and, to a lesser extent, testis. MSMP was highly expressed in PC3 cells, but not in 2 other prostate cancer cell lines. Western blot analysis revealed 20 kDa and 12 kDa bands PSMP maybe due to posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for MSMP antibody 19526-1-APDownload protocol
IF protocol for MSMP antibody 19526-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols