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MSRA Polyclonal antibody, PBS Only

MSRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14547-1-PBS

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Synonyms

验证数据展示

  • IF Staining of HEK-293 using 14547-1-AP (same clone as 14547-1-PBS)

    IF Staining of HEK-293 using 14547-1-AP (same clone as 14547-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14547-1-AP (MSRA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

  • WB analysis using 14547-1-AP (same clone as 14547-1-PBS)

    WB analysis using 14547-1-AP (same clone as 14547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 14547-1-AP (same clone as 14547-1-PBS)

    IP experiment of mouse kidney using 14547-1-AP (same clone as 14547-1-PBS)

    IP result of anti-MSRA (IP:14547-1-AP, 4ug; Detection:14547-1-AP 1:600) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

    IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

    IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

产品信息

14547-1-PBS targets MSRA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MSRA fusion protein Ag6053 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methionine sulfoxide reductase A
Synonyms
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 24-26 kDa
GenBank Accession NumberBC054033
Gene Symbol MSRA
Gene ID (NCBI) 4482
RRIDAB_2148366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJ68
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methionine sulfoxide reductase A(MSRA), is a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine.MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines.