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MT1A Polyclonal antibody

MT1A Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27115-1-AP

Print datasheet

Synonyms

metallothionein 1A, Metallothionein IA, MT 1A, MT IA, MT1, MT1A, MT1S, MTC

验证数据展示

  • IHC staining of human kidney using 27115-1-AP

    IHC staining of human kidney using 27115-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27115-1-AP (MT1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 27115-1-AP

    IHC staining of human kidney using 27115-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27115-1-AP (MT1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27115-1-AP

    IF Staining of HeLa using 27115-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MT1A antibody (27115-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27115-1-AP targets MT1A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen MT1A fusion protein Ag25747 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name metallothionein 1A
Synonyms metallothionein 1A, Metallothionein IA, MT 1A, MT IA, MT1, MT1A, MT1S, MTC
Calculated Molecular Weight 61 aa, 6 kDa
Observed Molecular Weight 6 kDa
GenBank Accession NumberBC029475
Gene Symbol MT1A
Gene ID (NCBI) 4489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04731
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for MT1A antibody 27115-1-APDownload protocol
IF protocol for MT1A antibody 27115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols