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MT3 Polyclonal antibody

MT3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 12179-1-AP

Print datasheet

Synonyms

GIF, GIFB, GRIF, Growth inhibitory factor, metallothionein 3, Metallothionein III, MT 3, MT III, MT3

验证数据展示

  • IHC staining of mouse brain using 12179-1-AP

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12179-1-AP

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12179-1-AP

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12179-1-AP

    IHC staining of mouse brain using 12179-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue, human brain tissue, human testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12179-1-AP targets MT3 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen MT3 fusion protein Ag2823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name metallothionein 3
Synonyms GIF, GIFB, GRIF, Growth inhibitory factor, metallothionein 3, Metallothionein III, MT 3, MT III, MT3
Calculated Molecular Weight 68 aa, 7 kDa
GenBank Accession NumberBC013081
Gene Symbol MT3
Gene ID (NCBI) 4504
RRIDAB_2146645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP25713
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MT3(Metallothionein-3) is also names as GIF(Growth inhibitory factor),GIFB inhibits N-glycosylation of IgE-binding factors and that unglycosylated IgE-binding factors selectively suppressed IgE synthesis.The major cell source of GIF is antigen-specific suppressor T(Ts)cell(PMID:10967025).The molecular size of GIF from T cells is 15 kDa, while GIF from macrophages consisted of 40-and 15-kDa molecules(PMID:2417235).

实验方案

Product Specific Protocols
IHC protocol for MT3 antibody 12179-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pig

Int J Mol Sci

Integrating Transcriptomics and Proteomics to Characterize the Intestinal Responses to Cadmium Exposure Using a Piglet Model

Authors - Yikun Li
View Article