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验证数据展示

  • WB analysis using 30545-1-AP

    WB analysis using 30545-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30545-1-AP (MTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 30545-1-AP

    IHC staining of human prostate cancer using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTA1 antibody included
  • IHC staining of human prostate cancer using 30545-1-AP

    IHC staining of human prostate cancer using 30545-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 30545-1-AP (MTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MTA1 antibody included
  • IF Staining of MCF-7 using 30545-1-AP

    IF Staining of MCF-7 using 30545-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MTA1 antibody (30545-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

MTA1 Polyclonal antibody

MTA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30545-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

MTA 1, metastasis associated 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NIH/3T3 cells, HEK-293T cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, human skin cancer tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy MTA1 Ready-To-Use IHC Kit. MTA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30545-1-AP targets MTA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 MTA1 fusion protein Ag30994 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 metastasis associated 1
别名 MTA 1, metastasis associated 1
计算分子量 81 kDa
观测分子量70-80 kDa
GenBank蛋白编号NM_004689
基因名称 MTA1
Gene ID (NCBI) 9112
RRIDAB_3086358
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13330
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Metastasis-associated protein 1 (MTA1) is a member of the MTA family which is involved in regulating DNA-based biological processes (PMID: 25332144). MTA1 is an integral part of nucleosome remodeling histone deacetylase complex (NuRD complex) and regulates nucleosome remodeling and deacetylation by stabilizing the NuRD complex and related proteins(PMID: 12419236). MTA1 expression is associated with tumor malignancy in several cancer.

实验方案

Product Specific Protocols
WB protocol for MTA1 antibody 30545-1-APDownload protocol
IHC protocol for MTA1 antibody 30545-1-APDownload protocol
IF protocol for MTA1 antibody 30545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations

Authors - Sihang Zhou
View Article