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MTA3 Polyclonal antibody
MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse thymus tissue, Jurkat cells, MCF-7 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14682-1-AP targets MTA3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MTA3 fusion protein Ag6400 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | metastasis associated 1 family, member 3 |
| Synonyms | mta1, MTA 3, Metastasis-associated protein MTA3 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 62 kDa, 68 kDa |
| GenBank Accession Number | BC053631 |
| Gene Symbol | MTA3 |
| Gene ID (NCBI) | 57504 |
| RRID | AB_2298003 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTC8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IHC protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IF protocol for MTA3 antibody 14682-1-AP | Download protocol |
| IP protocol for MTA3 antibody 14682-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell The polycomb group protein L3mbtl2 assembles an atypical PRC1-family complex that is essential in pluripotent stem cells and early development. | ||
Mol Cell The Nucleosome Remodeling and Deacetylation Complex Modulates Chromatin Structure at Sites of Active Transcription to Fine-Tune Gene Expression. | ||
Oncotarget Metastasis-associated protein 3 in colorectal cancer determines tumor recurrence and prognosis. | ||
PLoS One Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer.
| ||
J Gastroenterol Hepatol Overexpression of the metastasis-associated gene MTA3 correlates with tumor progression and poor prognosis in hepatocellular carcinoma. | ||
Tumour Biol MTA3 regulates malignant progression of colorectal cancer through Wnt signaling pathway. |
