验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HCT 116 cells, HT-29 cells, NIH/3T3 cells, C6 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83005-1-RR targets MTAP in WB, ELISA applications and shows reactivity with Human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse |
免疫原 | MTAP fusion protein Ag2051 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | methylthioadenosine phosphorylase |
别名 | c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase |
计算分子量 | 283 aa, 31 kDa |
观测分子量 | 31 kDa |
GenBank蛋白编号 | BC018625 |
基因名称 | MTAP |
Gene ID (NCBI) | 4507 |
RRID | AB_3670750 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q13126 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.
实验方案
Product Specific Protocols | |
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WB protocol for MTAP antibody 83005-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |