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MTBP Polyclonal antibody

MTBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28356-1-AP

Print datasheet

Synonyms

hMTBP, Mdm2 binding protein, MDM2BP, MTBP

验证数据展示

  • WB analysis of U2OS using 28356-1-AP

    WB analysis of U2OS using 28356-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28356-1-AP

    WB analysis using 28356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28356-1-AP (MTBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28356-1-AP

    IHC staining of human lung cancer using 28356-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28356-1-AP (MTBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inU2OS cells, K-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28356-1-AP targets MTBP in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen MTBP fusion protein Ag28393 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse) binding protein, 104kDa
Synonyms hMTBP, Mdm2 binding protein, MDM2BP, MTBP
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 38 kDa, 110 kDa
GenBank Accession NumberBC013136
Gene Symbol MTBP
Gene ID (NCBI) 27085
RRIDAB_2881118
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DY7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTBP antibody 28356-1-APDownload protocol
IHC protocol for MTBP antibody 28356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

Circular RNA Circ_0000119 promotes gastric cancer progression via circ_0000119/miR-502-5p/MTBP axis

Authors - Mengyan Xie
View Article