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MTCH2 Polyclonal antibody
MTCH2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (2)
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat testis tissue |
Positive IF/ICC detected in | U-251 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:14000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16888-1-AP targets MTCH2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, CoIP |
Tested Reactivity | human, rat |
Cited Reactivity | human, mouse, pig |
Immunogen | MTCH2 fusion protein Ag10399 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | mitochondrial carrier homolog 2 (C. elegans) |
Synonyms | MIMP, Met-induced mitochondrial protein, HSPC032 |
Calculated Molecular Weight | 303 aa, 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC000875 |
Gene Symbol | MTCH2 |
Gene ID (NCBI) | 23788 |
RRID | AB_2266733 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y6C9 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for MTCH2 antibody 16888-1-AP | Download protocol |
IHC protocol for MTCH2 antibody 16888-1-AP | Download protocol |
IF protocol for MTCH2 antibody 16888-1-AP | Download protocol |
IP protocol for MTCH2 antibody 16888-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology
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J Cell Biol The modified mitochondrial outer membrane carrier MTCH2 links mitochondrial fusion to lipogenesis. | ||
FASEB J MTCH2 promotes adipogenesis in intramuscular preadipocytes via an m6A-YTHDF1-dependent mechanism. | ||
RNA Biol Reprogramming of m6A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia. | ||
Am J Pathol miR-135b coordinates progression of ErbB2-driven mammary carcinomas through suppression of MID1 and MTCH2. | ||
Mol Med Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma.
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