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  • KD/KO Validated

MTCH2 Polyclonal antibody

MTCH2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 16888-1-AP

Print datasheet

Synonyms

MIMP, Met-induced mitochondrial protein, HSPC032



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat testis tissue
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16888-1-AP targets MTCH2 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, rat
Cited Reactivityhuman, mouse, pig
Immunogen MTCH2 fusion protein Ag10399 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial carrier homolog 2 (C. elegans)
Synonyms MIMP, Met-induced mitochondrial protein, HSPC032
Calculated Molecular Weight 303 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000875
Gene Symbol MTCH2
Gene ID (NCBI) 23788
RRIDAB_2266733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6C9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTCH2 antibody 16888-1-APDownload protocol
IHC protocol for MTCH2 antibody 16888-1-APDownload protocol
IF protocol for MTCH2 antibody 16888-1-APDownload protocol
IP protocol for MTCH2 antibody 16888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar
  • KD Validated
humanWB,IF

J Cell Biol

The modified mitochondrial outer membrane carrier MTCH2 links mitochondrial fusion to lipogenesis.

Authors - Katherine Labbé
pigWB

FASEB J

MTCH2 promotes adipogenesis in intramuscular preadipocytes via an m6A-YTHDF1-dependent mechanism.

Authors - Qin Jiang
humanWB

RNA Biol

Reprogramming of m6A epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia.

Authors - Yan-Jie Wang
mouseWB

Am J Pathol

miR-135b coordinates progression of ErbB2-driven mammary carcinomas through suppression of MID1 and MTCH2.

Authors - Arigoni Maddalena M
humanWB,IF

Mol Med

Inhibition of mitochondrial carrier homolog 2 (MTCH2) suppresses tumor invasion and enhances sensitivity to temozolomide in malignant glioma.

Authors - Qiuyun Yuan
  • KD Validated