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MTCO2 Recombinant antibody, PBS Only

MTCO2 Recombinant Antibody for WB, IP, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230448C9

Cat No : 83132-7-PBS

Print datasheet

Synonyms

COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2

验证数据展示

  • WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

    WB analysis using 83132-7-RR (same clone as 83132-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83132-7-RR (MTCO2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

    IP experiment of HeLa using 83132-7-RR (same clone as 83132-7-PBS)

    IP result of anti-MTCO2 (IP:83132-7-RR, 4ug; Detection:83132-7-RR 1:3000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

    IF Staining of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTCO2 antibody (83132-7-RR, Clone: 230448C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

    FC experiment of HepG2 using 83132-7-RR (same clone as 83132-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human MTCO2 (83132-7-RR, Clone:230448C9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83132-7-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MTCO2/COX2 Ready-To-Use IHC Kit. MTCO2/COX2 primary antibody included.

产品信息

83132-7-PBS targets MTCO2 in WB, IP, IF, FC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, IF, FC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytochrome c oxidase II
Synonyms COII, COX2, COXII, cytochrome c oxidase II, Cytochrome c oxidase subunit 2, MT CO2, MTCO2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberYP_003024029
Gene Symbol COX2
Gene ID (NCBI) 4513
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00403
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MTCO2, also named as COII, COXII, and COX2, belongs to the cytochrome c oxidase subunit 2 family. It is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Defects in COX2 are a cause of mitochondrial complex IV deficiency (MT-C4D).