验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, C2C12 cells, mouse liver tissue, rat kidney tissue, rat liver tissue |
| Positive IHC detected in | mouse cerebellum tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55082-1-AP targets MTCO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome c oxidase III |
| 别名 | COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | YP_003024032 |
| 基因名称 | MTCO3 |
| Gene ID (NCBI) | 4514 |
| RRID | AB_2881265 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P00414 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTCO3 antibody 55082-1-AP | Download protocol |
| IHC protocol for MTCO3 antibody 55082-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Diabetes Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis. | ||
Int J Mol Sci Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice. | ||
J Med Genet COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency. | ||
Sci China Life Sci Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival. | ||
