MTCO3 Polyclonal antibody

MTCO3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55082-1-AP

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Synonyms

COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3



经过测试的应用

Positive WB detected inmouse kidney tissue, C2C12 cells, mouse liver tissue, rat kidney tissue, rat liver tissue
Positive IHC detected inmouse cerebellum tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55082-1-AP targets MTCO3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome c oxidase III
Synonyms COIII, COXIII, cytochrome c oxidase III, Cytochrome c oxidase subunit 3, MT CO3, MTCO3
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 32-35 kDa
GenBank Accession NumberYP_003024032
Gene Symbol MTCO3
Gene ID (NCBI) 4514
RRIDAB_2881265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00414
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTCO3 (cytochrome c oxidase subunit III) is a mitochondrial DNA encoded subunit of respiratory Complex IV which is located within the mitochondrial inner membrane and is the final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. The predicted MW of MTCO3 is 30 kDa, while it had been observed as 23 kDa in some papers (PMID: 17557076).

实验方案

Product Specific Protocols
WB protocol for MTCO3 antibody 55082-1-APDownload protocol
IHC protocol for MTCO3 antibody 55082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Active RNA interference in mitochondria.

Authors - Kuanxing Gao
mouseWB

Diabetes

Transketolase Deficiency in Adipose Tissues Protects Mice From Diet-Induced Obesity by Promoting Lipolysis.

Authors - Na Tian
mouseWB

Int J Mol Sci

Mitochondrial rRNA Methylation by Mettl15 Contributes to the Exercise and Learning Capability in Mice.

Authors - Olga A Averina
humanWB

J Med Genet

COA7 (C1orf163/RESA1) mutations associated with mitochondrial leukoencephalopathy and cytochrome c oxidase deficiency.

Authors - Anabel Martinez Lyons
mouseIF

Sci China Life Sci

Cholesterol homeostasis regulated by ABCA1 is critical for retinal ganglion cell survival.

Authors - Jialiang Yang
humanWB

Life Sci Alliance

Drosophila MIC10b can polymerize into cristae-shaping filaments

Authors - Till Stephan