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  • Featured Product
  • KD/KO Validated

MTHFD1 Monoclonal antibody, PBS Only

MTHFD1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1G3

Cat no : 67670-1-PBS

Print datasheet

Synonyms

MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic

验证数据展示

  • WB analysis using 67670-1-Ig (same clone as 67670-1-PBS)

    WB analysis using 67670-1-Ig (same clone as 67670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67670-1-Ig (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

    IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTHFD1 antibody included
  • IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

    IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.

产品信息

67670-1-PBS targets MTHFD1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen MTHFD1 fusion protein Ag24553 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Synonyms MTHFC, Epididymis secretory sperm binding protein, C1-THF synthase, C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed, C-1-tetrahydrofolate synthase, cytoplasmic
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC009806
Gene Symbol MTHFD1
Gene ID (NCBI) 4522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)