• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

MTHFD1 Monoclonal antibody, PBS Only

MTHFD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1G3

Cat No : 67670-1-PBS

Print datasheet

Synonyms

MTHFC, MTHFD, MTHFD1

验证数据展示

  • WB analysis using 67670-1-Ig (same clone as 67670-1-PBS)

    WB analysis using 67670-1-Ig (same clone as 67670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67670-1-Ig (MTHFD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

    IHC staining of human colon cancer using 67670-1-Ig (same clone as 67670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67670-1-Ig (MTHFD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MTHFD1 antibody included
  • IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

    IF Staining of HEK-293 using 67670-1-Ig (same clone as 67670-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MTHFD1 antibody (67670-1-Ig, Clone: 2B1G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67670-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy MTHFD1 Ready-To-Use IHC Kit. MTHFD1 primary antibody included.

产品信息

67670-1-PBS targets MTHFD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen MTHFD1 fusion protein Ag24553 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Synonyms MTHFC, MTHFD, MTHFD1
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 101 kDa
GenBank Accession NumberBC009806
Gene Symbol MTHFD1
Gene ID (NCBI) 4522
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11586
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

MTHFD1 is a trifunctional enzyme involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism. MTHFD1 is associated with HCC and CRC progression. It has been reported that MTHFD-deficient patient fibroblasts exhibit enriched MTHFD1 in the nucleus which is critical for de novo dTMP synthesis.(PMID: 31133746, 30997850, 31421459, 25548164)