验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, COLO 320 cells, mouse ovary tissue, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16113-1-AP targets MTHFD1L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | MTHFD1L fusion protein Ag9036 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like |
| 别名 | MTC1THFS, Monofunctional C1-tetrahydrofolate synthase, mitochondrial, FTHFSDC1, EC:6.3.4.3 |
| 计算分子量 | 978 aa, 106 kDa |
| 观测分子量 | 106 kDa |
| GenBank蛋白编号 | BC017477 |
| 基因名称 | MTHFD1L |
| Gene ID (NCBI) | 25902 |
| RRID | AB_2250974 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6UB35 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTHFD1L(Monofunctional C1-tetrahydrofolate synthase, mitochondrial) is also named as FTHFSDC1(Formyltetrahydrofolate synthetase). MTHFD1L enzyme is present in mitochondria from normal embryonic tissues and embryonic fibroblast cell lines, and embryonic mitochondria possess the ability to synthesize formate from glycine. It catalyzes the final step in the mitochondrial conversion of 1-C units to formate in embryos. Moreover, MTHFD1L levels were substantially higher in embryonic mitochondria than in adult liver mitochondria and embryonic mitochondria exhibited greater formate production(PMID:19948730). It has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTHFD1L antibody 16113-1-AP | Download protocol |
| IHC protocol for MTHFD1L antibody 16113-1-AP | Download protocol |
| IF protocol for MTHFD1L antibody 16113-1-AP | Download protocol |
| IP protocol for MTHFD1L antibody 16113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions. | ||
Cell Metab Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability. | ||
Cell Metab Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism. | ||
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
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