MTHFR Monoclonal antibody, PBS Only

MTHFR Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7A3

Cat no : 66612-1-PBS

Print datasheet

Synonyms

Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3



产品信息

66612-1-PBS targets MTHFR in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen MTHFR fusion protein Ag14970 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name 5,10-methylenetetrahydrofolate reductase (NADPH)
Synonyms Methylenetetrahydrofolate reductase (NADPH), EC:1.5.1.53, 1F7A3
Calculated Molecular Weight 656 aa, 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC053509
Gene Symbol MTHFR
Gene ID (NCBI) 4524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42898
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

5,10-Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in the folate metabolic pathway with a key role in generating methyl groups. MTHFR reduces 5,10-methylenetetrahydrofolate (5,10-methyleneTHF) to 5-methylTHF, the primary carbon donor for methionine production from homocysteine. In humans and mice studies, mutations and polymorphisms in MTHFR are associated with impaired spermatogenesis and male infertility. It has been reported that MTHFR is highly phosphorylated under unperturbed conditions and T34 is the priming phosphorylation site. MTHFR gene has two promoters and isoform (70 kDa and 77 kDa). Although the major form of MTHFR is 77kDa in most tissues, a second isoform of 70kDa was observed after western blotting of human fetal liver and porcine liver. (PMID: 24769206, PMID: 34128976, PMID: 18615588)