验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, human preadipocyte cells, HT-29 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver tissue, mouse testis tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
14219-1-AP targets MTIF3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | MTIF3 fusion protein Ag5457 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | mitochondrial translational initiation factor 3 |
| 别名 | IF 3(Mt), IF 3mt, IF3(mt), IF3mt, MTIF3 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC046166 |
| 基因名称 | MTIF3 |
| Gene ID (NCBI) | 219402 |
| RRID | AB_10638621 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2K0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MTIF3, also named as DC38, belongs to the IF-3 family. MTIF3 encodes a 29 kDa protein that promotes formation of the initiation complex on the mitochondrial 55S ribosome, thereby playing an active role in initiation of translation. Like bacterial IF3, MTIF3 is believed to bind first to the small mitoribosomal subunit to keep it dissociated from the large subunit during initiation. After binding of MTIF3, mRNA and formylated initiator methionyl-tRNA (fMet-tRNAifMet) bind to the small mitoribosomal subunit. The large subunit then joins the small subunit to form an elongation-competent ribosome (PMID:20887776, 31350787).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MTIF3 antibody 14219-1-AP | Download protocol |
| IHC protocol for MTIF3 antibody 14219-1-AP | Download protocol |
| IP protocol for MTIF3 antibody 14219-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv Fidelity of translation initiation is required for coordinated respiratory complex assembly. | ||
EMBO J Stress signaling and cellular proliferation reverse the effects of mitochondrial mistranslation. | ||
Sci Rep Initiation Factor 3 is Dispensable For Mitochondrial Translation in Cultured Human Cells. | ||
Sci Total Environ Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects | ||
Elife Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function |
