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MTM1 Polyclonal antibody

MTM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13924-1-AP

Print datasheet

Synonyms

Myotubularin, MTMX, EC:3.1.3.95, EC:3.1.3.64, CNM

验证数据展示

  • WB analysis of L02 using 13924-1-AP

    WB analysis of L02 using 13924-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 13924-1-AP (MTM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skeletal muscle using 13924-1-AP

    IHC staining of rat skeletal muscle using 13924-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat skeletal muscle using 13924-1-AP

    IHC staining of rat skeletal muscle using 13924-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 13924-1-AP (MTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of L02 using 13924-1-AP

    IF Staining of L02 using 13924-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using MTM1 antibody (13924-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, Jurkat cells
Positive IHC detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13924-1-AP targets MTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitypig
Immunogen MTM1 fusion protein Ag4940 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myotubularin 1
Synonyms Myotubularin, MTMX, EC:3.1.3.95, EC:3.1.3.64, CNM
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC030779
Gene Symbol MTM1
Gene ID (NCBI) 4534
RRIDAB_2147700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13496
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The MTM1(Myotubularin) gene encodes a protein that belongs to a family of putative tyrosine phosphatases. It is required for muscle cell differentiation. The protein is also a potent phosphatidylinositol 3-phosphate (PI3P) phosphatase, which is a 66 kDa ubiquitous protein that is found mutated in the X-linked genetic disorder myotubular myopathy(XLMTM).MTM1 could therefore act as a regulator of the highly active phosphoinositide (PI) metabolism in cells, where interconversions of the eight PIs described so far is controlled by a set of specific kinases and phosphatases(PMID:14660569). MTM1 has two isoforms with the molecular mass of 66kDa and 70 kDa.

实验方案

Product Specific Protocols
WB protocol for MTM1 antibody 13924-1-APDownload protocol
IHC protocol for MTM1 antibody 13924-1-APDownload protocol
IF protocol for MTM1 antibody 13924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

PLoS Pathog

Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry

Authors - Bing-Qian Su
View Article