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MTMR12 Polyclonal antibody

MTMR12 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 17552-1-AP

Print datasheet

Synonyms

3 PAP, 3 phosphatase adapter protein, KIAA1682, MTMR12, PIP3AP

验证数据展示

  • WB analysis using 17552-1-AP

    WB analysis using 17552-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 17552-1-AP

    WB analysis of HEK-293 using 17552-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 17552-1-AP

    IP experiment of K-562 using 17552-1-AP

    IP result of anti-MTMR12 (IP:17552-1-AP, 4ug; Detection:17552-1-AP 1:500) with K-562 cells lysate 2400 ug.

经过测试的应用

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue, mouse lung tissue
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17552-1-AP targets MTMR12 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen MTMR12 fusion protein Ag11710 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myotubularin related protein 12
Synonyms 3 PAP, 3 phosphatase adapter protein, KIAA1682, MTMR12, PIP3AP
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC057393
Gene Symbol MTMR12
Gene ID (NCBI) 54545
RRIDAB_2147824
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0I1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MTMR12 antibody 17552-1-APDownload protocol
IP protocol for MTMR12 antibody 17552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

FASEB Bioadv

In search of alternatively spliced alpha-Klotho Kl1 protein in mouse brain

Authors - Liping Li
View Article