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验证数据展示

  • WB analysis using 14973-1-AP

    WB analysis using 14973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14973-1-AP (MTMR14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14973-1-AP

    IF Staining of HeLa using 14973-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MTMR14 antibody (14973-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Featured Product
  • KD/KO验证

MTMR14 Polyclonal antibody

MTMR14 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14973-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Myotubularin-related protein 14, hJumpy, hEDTP, HCV NS5A-transactivated protein 4 splice variant A-binding protein 1, EC:3.1.3.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells, mouse testis tissue, rat testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14973-1-AP targets MTMR14 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 MTMR14 fusion protein Ag6883 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 myotubularin related protein 14
别名 Myotubularin-related protein 14, hJumpy, hEDTP, HCV NS5A-transactivated protein 4 splice variant A-binding protein 1, EC:3.1.3.-
计算分子量 72 kDa
观测分子量60-72 kDa
GenBank蛋白编号BC050626
基因名称 MTMR14
Gene ID (NCBI) 64419
RRIDAB_2147828
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCE2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MTMR14, also known as Jumpy, belongs to the MTM1/MTMRs family which has 15 members that divided into two subgroup: catalytically inactive and active. MTMR14 is a catalytically active member possessing an active phosphatase domain, that specifically dephosphorylates PI3P and phosphatidylinositol (3,5)-bisphosphate (PI3,5P2) at position 3 on inositol ring. A remarkable feature of the MTM1/MTMRs family is the association between a catalytically inactive member and a catalytically active member, which plays an important regulatory role. MTMR14 has 3 isoforms with molecular weight of 60-72 kDa.

实验方案

Product Specific Protocols
WB protocol for MTMR14 antibody 14973-1-APDownload protocol
IF protocol for MTMR14 antibody 14973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Gene

Knockdown MTMR14 promotes cell apoptosis and inhibits migration in liver cancer cells.

Authors - Zhaodong Li
  • KD Validated
View Article